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After incubation, the preabsorbed antibody was reacted with the spinal cord section for incubation overnight at 4C

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Infection warning signs: Timing: Symptoms appear or worsen 2 to 5 days after injection (delayed compared to reaction symptoms) Redness: Progressively expanding, bright red, with distinct borders Pain: Increasing rather than decreasing over time Warmth: Injection site feels significantly warmer than surrounding skin Drainage: Yellow or green pus, cloudy fluid, or foul odor Systemic: Fever, chills, general malaise If you suspect infection, do not attempt to treat it at home with warm compresses or over-the-counter antibiotics