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TNB undergoes a further ionization in neutral and alkaline pH in water to form the yellow-colored TNB 2 species, the absorbance of which is quantified using a plate reader at 412 nm

However, the committee also recognizes the importance of stability in regulatory policy to the regulated community and thus suggests that EPA establish criteria for identifying when compelling new information warrants science-based revisions in its risk assessment

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The final step, in which the completed peptide is released from the polymer support, is a simple benzyl ester cleavage

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