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RNA (2 g) was reverse transcribed to cDNA using the SuperScript II RNase H-reverse transcriptase system (Invitrogen, Carlsbad, CA, USA)
B) The amount of NO produced is directly related to nitrite concentration, as is evident from the high levels produced even at pH 7 when nitrite concentration is very high.
Zhuang JS, Chen QL, Xu LY, Qiao DF, Chen XB
Excess ROS then activates the NLRP3 inflammasome in microglia, promoting maturation and release of IL-1 (Xiao et al., 2021)
Fry et al., 2001)
