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Unlike benzodiazepines or other sedatives, it doesn't appear to create tolerance requiring escalating doses or produce withdrawal symptoms upon cessation
7 Then came the pivotal test
Proc Natl Acad Sci USA 111:1326413271
Metaphase I (M) oocytes were fixed in 4% (w/v) paraformaldehyde for 15 min at room temperature and then washed three times with DPBS for 3 min each time
This thiol can be further removed to produce a double bond at 6 th position to yield an important metabolite Canrenone
