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massiliensis were then extracted from quality-filtered reads by using blastn algorithm [38] ( e value 50% stenosis for at least one coronary vessel) were recruited as an independent cohort for validating the microbiome-based TMAO producer prediction model (the baseline characteristics of CVD validation cohort were described in Table S2)

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In addition, carbonyl groups ( C = O) and phosphates were added as signatures of increased hydrolysis

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