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PCR primers were designed to keep the first and last 24 nucleotides (8 amino acids), respectively, of the gshT coding sequence intact to reduce the possibility of downstream effects

Compared to the single nanoparticle used, the combined nanoparticle-hydrogel possesses a high risk in terms of safety, and therefore, it must be systematically investigated

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A separate line of investigation, work by Pollard and colleagues, demonstrated that GHK-Cu could reverse detrimental changes in irradiated cultured human fibroblasts: cells exposed to significant radiation and then treated with GHK-Cu at very low concentrations displayed a growth pattern and growth-factor secretion profile comparable to non-irradiated control cells, including increased secretion of bFGF and VEGF