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To construct the P arqI reporter pCC21-P arqI -mScarlet-I, the 145 bp upstream regulatory region of ArqI was PCR amplified using primer pairs SacI-P arqI -F and XbaI-NcoI-P arqI -R ( SI ), followed by digestion with SacI and XbaI (New England Biolabs) to produce complementary overhangs
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Romeo A, Kazsoki A, Musumeci T, Zelko R
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