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Localization of Ctrs by Fluorescence Microscopy For protein localization, wild-type (WT) yeast BY4741 cells were transformed with pUG35 containing either the NgCTR or NfCTR gene, and transformants were grown overnight at 30C in liquid SC-ura medium, washed and resuspended in phosphate-buffered saline (PBS), pipetted onto a microscope slide and mixed with the same volume of 2% agarose

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[DOI] [PMC free article] [PubMed] [Google Scholar] 128.Griffiths P.D., Dobson B.R., Jones G.R., Clarke D.T