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The proton-motive force created by the ETC is also used for many additional mitochondrial processes, especially those related with transport across the IMM ( Although the primary function of mitochondria is to generate ATP as an energy molecule required for countless cell functions, a small fraction of electrons from the ETC are transferred directly to O 2 , resulting in the generation of the superoxide anion, which can give rise to other ROS as well as reactive nitrogen species (RNS)

[DOI] [PMC free article] [PubMed] [Google Scholar] 78.Bi Y., Li H., Yi D., Sun Y., Bai Y., Zhong S., Song Y., Zhao G., Chen Y

10.1111/jgs.16256 86

Parade DS

Therefore, the robust assignment of ferroptosis should involve coupling of lipid-peroxidation readouts with rescue by lipophilic radical-trapping antioxidants or iron chelators and evidence of GPX4/system Xc contribution