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[DOI] [PubMed] [Google Scholar] 21.Atit R, Sgaier SK, Mohamed OA, Taketo MM, Dufort D, Joyner AL, Niswander L, Conlon RA

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Objective EEG-based metrics showed faster sleep initiation and more continuous sleep with saffron: sleep-onset latency fell about 21% compared with an approximate 19% rise with placebo, latency to persistent sleep dropped about 28% versus a roughly 35% rise, and wake after sleep onset decreased (6.1% vs

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Relative expression was determined by the 2 Ct method using the housekeeping gene, GAPDH, as an internal control, and the fold change was calculated by its comparison with the corresponding control group [28]