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Cells were rinsed with PBS, stained with DAPI for 5 min, and mounted using Prolong Gold Antifade Reagent

In addition to the intra- and intermolecular hydrogen bonds of these structures, keratins have large amounts of the sulfur-containing amino acid Cys, resulting in disulfide bridges that confer additional strength and rigidity

Glutathione is a powerful antioxidant that helps brighten skin by inhibiting the enzyme tyrosinase, which is responsible for melanin production

A meta-analysis and systematic review

If this subtle approach does not resolve the problem, other possible approaches include changes in the pharmaceutical form for oral administration, weekly oral ingestion of LT4 under the supervision of medical staff, or exceptionally (and preferably at an expert centre) the parenteral infusion of LT4