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For in vitro study, the cells were fixed with 4% paraformaldehyde in phosphate buffer

This condition also induces an increase in several inflammatory mediators, including the protein levels and mRNA expression of TLR-4, NF-B, p65, and p38 MAPK, as well as elevated levels of proinflammatory cytokines such as IL-1, ICAM-1, and TNF- [ 51 , 52 ]

doi: 10.1186/1477-7819-11-235

While these DMVs are generally believed to be formed via viruses manipulating the endoplasmic reticulum (ER) membrane, the mechanism for importing and packaging proteins and RNA into these miniature organelles is not clearly understood [68]

Sasaki K, Kantarjian HM, Ravandi F, Thomas DA, Cortes JE, Pemmaraju N, Kadia TM, Short NJ, Issa GC, Garris RS, Jeanis V, Moore HG, Garcia-Manero G, Borthakur G , Wierda WG, Gachimova E, O'Brien SM, Jabbour E