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ATCC authenticated by STR profyling) and grown in DMEM medium (Gibco) supplemented with 10% FCS, 1% penicillinstreptomycin solution (Gibco) under humidified atmosphere of 95% air/5% CO 2 at 37 C

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The result of worsened outcome due to chronic stress could be the result of activation of HPA (hypothalamic-pituitary-adrenal), as well as SNS (sympathetic nervous system) (Nusslock and Miller, 2016), and microglial activation (Kosten et al., 2012

Microbial burden Ageing is correlated with a reduced abundance of salutary commensal microbes that maintain the intact intestinal barrier and limit the amplification of pathogenic commensals by secretion of specific metabolites, for instance, the short-chain fatty acids

Burden Associated With Submission of Additional Information on the Action and Transition Plans for Enrollment as an REH We are proposing that an eligible facility that submits an application for enrollment as an REH under section 1866(j) of the Act must also submit additional information as specified in this proposed rule