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[DOI] [PubMed] [Google Scholar] 92.Duarte PM, Napimoga MH, Fagnani EC, Santos VR, Bastos MF, Ribeiro FV, et al

Inoculate several isolated colonies into separate 50 ml centrifuge tubes containing 10 ml LB supplemented with 100 g/ml ampicillin

Most treatments take 3060 minutes , depending on the dosage and individual needs

However, it is important to note that caution should be exercised when considering high coffee consumption, as excessive consumption, particularly due to diterpenes, has been associated with elevated cholesterol levels, posing potential risks to coronary health [277]