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Clin J Sport Med, 16:422-425

Critical review of exposure and effects: implications for setting regulatory health criteria for ingested copper

GSH assay fluorescence was determined with a fluorescence spectrophotometer at 420 nm (excitation at 350 nm) after incubating 0.2 ml of supernatant with 1.7 ml of potassium phosphate-EDTA buffer (pH 8.0) and 0.1 ml of fluorescence reagent o -phthaldialdehyde (1 mg/ml) for 15 min

Aoyama K, Matsumura N, Watabe M, Wang F, Kikuchi-Utsumi K, Nakaki T

The timing question for GHK-Cu resolves to a simple biochemical fact: the peptide works when growth hormone receptors are available to receive it