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The Clustal omega program was employed to perform protein sequence alignment ( Phylogenetic trees were constructed in MEGA 11, by the Neighbor-joining method using 1000 bootstraps as the phylogeny test for Gpx proteins, and by the Maximum Likelihood method using 500 bootstraps as the phylogeny test for Gst proteins

[1] The CDC states that diagnosing ME/CFS relies on a physical and psychiatric assessment, observations, medical history, clinical interview, and results from a mix of blood and urine tests