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Disulfide-containing linkers are primarily linked to maytansinoid payload classes, and the reactivity of disulfide bonds can be regulated by steric hindrance: alpha methyl substitution significantly affects reduction rate and resistance to thiol-disulfide exchange force

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When the structural information of the enzyme protein is known, computer-aided analysis of the conserved sites and crystal structure could be used to select several amino acid sites as the engineered targets in a non-random way
