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diluted 1:100 in RPMI 1640) for nuclear staining

Generally, iron accumulation induces OS, bringing about the increase of ROS levels and the imbalance of antioxidant balance and infiltrating pro-inflammatory mediators that further amplify cerebral inflammatory responses, which aggravate neuronal damage during reperfusion (Jin et al., 2013

You are checking for clarity, meaning you should be able to see through the liquid clearly

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