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Quantitative real-time PCR Total RNAs were extracted by Trizol (Invitrogen) and dissolved in an appropriate amount of RNase-free water

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Li X, Wang H, Shimizu Y, Pyatenko A, Kawaguchi K, Koshizaki N (2011) Preparation of carbon quantum dots with tunable photoluminescence by rapid laser passivation in ordinary organic solvents
