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These metabolites can be broadly classified into water-soluble antioxidantssuch as proline, glycine betaine, ascorbic acid (AsA), and glutathione (GSH)and lipophilic antioxidants, including tocopherols and carotenoids, reflecting both their chemical properties and intracellular compartmentalization (Mishra et al

doi: 10.1211/jpp.59.8.0010

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The liver, which houses the highest glutathione concentrations and performs extensive detoxification work during sleep, may benefit from elevated glutathione availability during these nighttime processes

quantification of proteins was carried out with the BCA reagent