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Post-fixation of the cells after the secondary antibody is applied can also reduce potential lipid movement as the affixed antibody can be crosslinked to neighboring proteins

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Diabetes Care 42(11):e172-e4 2019 PMC6804608 Powell J, Isom S, Divers J, Bellatorre A, Johnson M, Smiley J, Begay Q, Benally C, Hu D, Saydah S, Pettitt DJ, Pihoker C, Dabelea D, SEARCH for Diabetes in Youth Study

[DOI] [PMC free article] [PubMed] [Google Scholar] Schellekens AF, Mulders PC, Ellenbroek B, et al