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The degree of hemolysis was assessed using an enzyme marker at 540 nm
These are consistent with the class of compounds it belongs to and often lessen over time
While GHSR1a constitutive activity is required for amplification of MC3R signaling, it has minimal effect on GHSR1a-MC3R dimer internalization, as treatment with the GHSR1a inverse agonist SP-analog only results in a small, nonsignificant reduction in internalization (Schellekens et al., 2013)
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(2004) Accelerated telomere shortening in response to life stress
