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Description
The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)

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Heavy metal contamination, residual solvents, and other manufacturing impurities pose additional health risks unrelated to the peptides themselves
Warnings and Precautions Pregnancy and breast-feeding: Vitamin C is likely safe for pregnant or breast-feeding women when taken by mouth in amounts no greater than 2000mg daily for women over 19 years old, and 1800mg daily for women 14-18 years old, or given intramuscularly or intravenously

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