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At the definite time points, cells were lysed with RIPA buffer (10 2 M TrisCl (pH 8.0), 10 3 M EDTA, 5 10 4 M EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 0.14 10 3 M NaCl, 1 protease inhibitor cocktail (#P8340), 1 phosphatase inhibitor cocktail 2 and 3 (#P5726, P0044), 10 3 M PMSF (all obtained from Sigma-Aldrich)

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