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Description
Secondary antibodies (anti-rabbit IgG or anti-mouse IgG, 1:2000) were purchased from Cell Signaling Technology
Cells were incubated with PEI-Coated-MNPs-CRISPR/Cas9 plasmid complexes (1 g DNA/well) in serum free media for 30 min followed by addition of 10% FBS via magnetic assisted transfection (magnetofection), in which a magnet was placed underneath the cells for 1 h to facilitate the localization of MNPs to the adhered cells during in vitro gene delivery
(2006) compared different groups of RAW 264.7 cell line murine macrophages that treated with diallyl trisulfide, diallyl disulfide, and diallyl sulfide, respectively
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