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Nat Commun 11: 6285, 2020
doi: 10.1515/revneuro-2017-0040

IPSC, Induced Pluripotent Stem Cell

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Advantages and disadvantages when using one-step versus two-step assays in RT-qPCR Reverse transcription in RT-qPCR Choosing total RNA vs mRNA When designing a RT-qPCR assay it is important to decide whether to use total RNA or mRNA as the template for reverse transcription