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However, due to the relatively small number of mast cells typically found in most tissues, phagocytosis is only a small piece of the puzzle when it comes to the role mast cells play in pathogen elimination

So long as they had been properly stored and there were no signs of deterioration then they were safe to use

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Rapid membrane disruption by a perforin-like protein facilitates parasite exit from host cells

Real-Time Quantitative Reverse Transcription PCR (qRTPCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 g of total RNA was reverse-transcribed into cDNA