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Yet another example of the undesirable effect of alcohol-induced hepatic hypo-methylation involves protein phosphatase-2A and the activity of Forkhead box O1 (FOXO1), a transcription factor that contributes to hepatic lipid metabolism

Z., & Rink, L

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718 Screening of this library led to the identification of a Tyr189Arg mutant that can incorporate the unnatural substrate with twice the efficiency into 6-deoxyerythronolide B ( 99 ) over the native methylmalonyl-CoA

The proteins were detected with SuperSignal West Pico PLUS chemiluminescence substrate (Thermo Fisher Scientific) and visualized with an iBright Western Blot Imaging System (Thermo Fisher Scientific)