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The Phase 1 results were divided into two groups: Group 1 (0 min) and Group 5 (pool prepared from samples collected at 0 + 30 + 60 min)

Zhao S, Rees JK, Andreeff M

Kumar A, Maini K, Kadian R

The following primers, also purchased from Eurogentec, were used either for the amplification during SELEX or for the final amplification step before cloning: 5-ATACCAGCTTATTCAATT-3 with and without 5-6-Carboxyfluorescein (FAM) and 5-AGATTGCACTTACTATCT-3 with and without 5-poly-A20-hexaethylene glycol spacer (HEGL)

(5) suggested that low levels of expression of this enzymatic protein are considered to have a significant role in sperm physiology and led to a reduction in functional competence of the sperm in low-fertility bulls