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Lysine residues are more abundant than cysteine, so typically you will get redundant biotinylation when using NHS Esters to label lysine residues

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doi: 10.2174/1567205013666160229112911

Electroanalysis 27(8):19891997 Shanker Sharma H, Sharma A (2012) Neurotoxicity of engineered nanoparticles from metals
