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GS is responsible for triggering the reaction that synthesizes glutamine from ammonium ion (NH 4+ ), and glutamate through ATP consumption, whereas GLS is responsible for glutamine hydrolysis, which converts it into glutamate and NH 4+ again [22,23] (Figure 1)

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In brief, 5 L of overnight culture in YNB was transferred to wells of 96-well microtiter plates containing 95 L of YNB medium supplemented (e.g

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Wei Q, Ren Y, Zheng X, et al