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Traditionally, in vitro liver studies have mainly relied upon two-dimensional (2D) cell culture models, by using primary hepatocytes or immortalized cell lines such as HepG2 and HepaRG cells

for example TP53 deletion in fibroblasts is found to diminish senescence and to increase TGF-1 expression, leading to enhanced activated fibroblasts, more extracellular matrix deposition, reduced immune surveillance, and increased fibrosis [66]

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The vascular structure of the respiratory region helps the nanoparticles to enter the bloodstream and thus indirectly the central nervous system[165] (Fig

Jung, Potential role of thymosin Beta 4 in liver fibrosis