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Beyond energy conversion, mitochondria are indispensable for a myriad of other cellular processes, including heme and steroid hormone synthesis, calcium ion (Ca 2+ ) buffering to regulate intracellular signaling, and the initiation of apoptosis via the mitochondrial pathway (36)

The 1821 gauge needle is common for drawing from a BAC water vial because the large bore makes it easy to pull significant volume quickly

Maintaining sterile technique is essential when working with bacteriostatic water
