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After that, the absorbance of the supernatant was read at 532 nm using a plate reader

Similar to EE, ICA at the range of 1 to 5 M enhanced the abundance of all types of MyHC isoforms and peaked at 2 M (Fig

doi: 10.1089/ars.2013.5745 118 RuppersbergJ

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GSH is the most abundant non-protein thiol in mammalian cells
