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Upon reaching 80% confluence, the cells were stimulated with 100 ng mL 1 LPS for 12 h to induce inflammation, which was confirmed by the overexpression of vWF on the inflamed HUVECs

Hence, the loss of these blebs after treatment by 1.5 mM SIN-1 might be a sign of inhibited surface transformation in vitro

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While the etiology of NTD is multifactorial, some studies have found that blood concentrations of homocysteine or holo-transcobalamin to be lower in NTD-affected infants and mothers compared to non-affected controls (reviewed in 59)