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Description
The plates were briefly stirred and the absorbance was monitored in kinetic mode in a plate reader SpectraMax ABS Plus (Molecular Devices)

Reactive oxygen species, apoptosis, and mitochondrial dysfunction in hearing loss

Having injection site reactions doesn't mean you're more likely to have dangerous allergic responses to the medication

Oncotarget 9 , 44854495 (2018)

Yes, it may cause constipation as one of its side effects
