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Because of the lack of catalase in mitochondria, H 2 O 2 metabolism is mediated through glutathione, with participation of GSHPX or peroxiredoxin

The therapeutic activity of NAC relies on its ability to provide reducing sulfhydryl moieties and to act as a precursor of GSH to replenish the intracellular GSH pool when this has been depleted in conditions of oxidative stress, in processes of drug detoxification, or in other conditions leading to GSH deficit

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Current and emerging therapies in the management of hypoxic ischemic encephalopathy in neonates

Importantly, iodoacetamide (IAA, a known thiol-reactive compound) was not able to oxidize roGFP when cells were exposed to non-lethal or subtoxic concentrations of this compound, indicating that an electrophilic attack on roGFP is not sufficient to cause a spectral change consistent with its oxidation (Supplementary Fig