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Methods Human neutrophil isolation Human neutrophils were isolated from normal donor blood by Ficoll density centrifugation, as previously described 67

To minimize the clash score further, individual chains of the refined model were used to split the EM map in UCSF Chimera, and the models of individual chains/subunits were subjected to flexible fitting and refinement using an online tool called Namdinator and Phenix (Namdinator- Automatic Molecular Dynamics Flexible fitting of structural models into cryoEM and crystallography experimental maps)