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AKT2 (RAC-), which acts in the insulin signaling pathway, is encoded on chromosome 19
2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added
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About the Author: Joseph Tauro, MD, a Board-Certified orthopedic surgeon, is nationally recognized for his expertise in treating shoulder, sports, and degenerative joint disorders
