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Cells were then washed twice with Hanks Balanced Salt Solution (HBSS, H8264) and incubated with 200 L GDC (12 concentrations, 05 mM) in DMEM D1145 or 1% TritonX (X-100)

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Trimix, which is an injectable combination of three vasodilating medications (papaverine, phentolamine, and prostaglandin E1), is a newer ED solution for men who dont respond adequately to oral medications