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Samples were washed twice with 1 X PBS, once with the CL-WS1 buffer [50 mM HEPES/KOH pH 7.5, 150 mM NaCl, 0.1% sodium deoxycholate, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF, EDTA-free protease inhibitor cocktail], ground in liquid nitrogen, resuspended in 2 ml ice-cold CL-Lysis buffer [50 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.1% sodium deoxycholate, 1% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM PMSF, EDTA-free protease inhibitor cocktail], and sonicated using a Bioruptor UCD-200 at high mode for 15 cycles of 30 s on and 30 s off

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The more common presentation in the neonatal period is miliaria crystallina, which appears as delicate, tiny, clear superficial vesicles, most commonly on the forehead

On the other side, our results clarified that co-administration of either LC, LCLN, QT or QTLN with ATZ dramatically improved semen quality and serum hormonal profile when compared to the ATZ group