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Borderline personality disorder BPD is not a mitochondrial diagnosis, and psychotherapy remains the core treatment

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This frustrating plateau is frequently tied to declining mitochondrial function, and MOTS-c therapy offers a targeted way to reignite metabolic efficiency at the cellular level

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The fixed organoid cultures were blocked in 5% donkey serum (Millipore, Billerica, MA, USA) + 0.3% Triton-X-100/PBS, incubated overnight in 3% bovine serum albumin (Sigma-Aldrich, St Louis, MO) + PBS with primary antibodies, washed, incubated with Alexa Fluor-conjugated secondary antibodies (Invitrogen, Carlsbad, CA), washed, and stained with DAPI or mounted in VECTASHIELD H-1000