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For Mounjaro: confirmed type 2 diabetes diagnosis, recent HbA1c, current medication list, documentation of metformin trial (unless contraindicated), and the planned dosing schedule

this process involved denaturation, enzyme reaction, and thermal cycling to produce stable complementary DNA for subsequent analysis

Mice were initially group housed (three/cage) and allowed to acclimate to the colony environment maintained at a constant temperature (2123C) and humidity (4050%) on a 12-hour light-dark cycle (lights on 06001800 h)