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After synchronization, N2 nematodes were cultured at 20C to the L4 stage and transferred to NGM medium with or without EEGE (0.5 or 1 mg/ml), with three parallel plates in each group and at least 15 nematodes in each plate
notably, this is the only NAD + synthesis pathway that operates independently of niacin [17]

You have multiple mitochondria in each cell (sometimes thousands of mitochondria)

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