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In addition, biological processes that depend on O 2 and nitrogen have gained greater importance because their end-products are usually found in states of high metabolic requirements, such as pathological processes or external environmental interactions [2]

Then sample copy number was computed with standard curve and normalized with GAPDH

All surgical procedures were conducted under general anesthesia, induced via intramuscular injection of 50 mg/kg ketamine hydrochloride (500 mg/10 mL
