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Description
Sections were cut with 4-m thickness using a rotary microtome, mounted on slides, and dried at 60 C

DOI: 10.1038/mi 37

The synthesis and characterization of compounds 112 and PRP are given in Supplementary Methods, Supplementary Figs

Enrichment of phosphopeptides by Fe immobilized metal ion affinity chromatography (IMAC) The remaining 95% was concatenated into 12 fractions and subjected to Fe 3+ -IMAC phosphopeptide enrichment using Fe 3+ -conditioned Ni-NTA beads

However, it is not fully characterized at the molecular level, and its contribution to lysine metabolism is unclear [27]