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To prepare protoplasts, Arabidopsis rosette leaves were digested with enzyme solution (1.5% cellulose R10, 0.4% macerozyme R10, 0.4 M mannitol, 20 mM KCl, 20 mM MES, 10 mM CaCl 2 , and 0.1% BSA) for 5 h
Cancer cells demand lipid uptake providing energy for their growth and survival [88, 89]
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