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For saturation binding assays, cell lysates (100 L, 25 g of protein for HEK-293 cells and 1020 g of protein for INS-1E cells, quantified by the Bradford Assay) were incubated with the indicated concentrations of [ 3 H]RX821002 (90 min, room temperature)
TVCL = 0.0329 L/h/70 kg
10.2174/1381612824666180614082950 [DOI] [PubMed] [Google Scholar] Kato N., Kawai T., Maeda H., Nagao T., Murakami H., Miyazawa K., et al
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