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Another study exposed HBECs in an ALI to aerosols of either nicotine-containing or nicotine-free e-liquids, finding that nicotine did not reduce cell viability at 4 h but did produce significant reductions in ciliary beat frequency and cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel conductance (46)
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doi: 10.3389/fonc.2021.729103 22 ParkKJKimJTestoffTAdamsJPoklarMZborowskiMet al
Additionally, pre-, inter-, and post-administration urine samples were collected until study day 33
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