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Samples were separated using a Kinetex 2.6 m XB-C18 100 , 100 2.1 mm LC Column (Phenomenex) with a constant flow rate of 250 l/min following a flow gradient starting at 70% Mobile Phase B (ACN + 0.5% Acetic acid, AA) then going up to 99% for 5 min before re-equilibration at 70% for 5 min

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Therefore, stabilization of mitochondria following antioxidant treatment could attenuate inflammatory processes and decrease the reaction of microglia and macrophages in the injured spinal cord

These findings support a causal role of mitochondrial bioenergetics in neurobehavioral phenotypes (Chauhan et al., 2011

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